Journal: The Journal of Headache and Pain

Article Title: Repeated oxytocin prevents central sensitization by regulating synaptic plasticity via oxytocin receptor in a chronic migraine mouse model

doi: 10.1186/s10194-021-01299-3

Figure Lengend Snippet: Effect of OT on synapse-associated protein expression levels. A Representative WB bands showing the expression of PSD-95, syt-1, and snap25. B , C , and D Quantification of PSD-95 ( B ), syt-1 ( C ), and snap25 ( D ) protein levels showed that these proteins were significantly increased in the NTG + vehicle group compared to the sham group. The expression levels of these three proteins after OT treatment were reduced, while L368,899 blocked these effects. One-way ANOVA with Dunnett’s post hoc test, n = 6/group; *** p < 0.001 vs. the sham group; ### p < 0.001 vs. the NTG + vehicle group; && p < 0.01, &&& p < 0.001 vs. the NTG + OT group

Article Snippet: The membranes were blocked for 2 h at room temperature in Tris-buffered saline with Tween-20 (TBST) containing 5 % nonfat milk and incubated overnight at 4 °C with the following primary antibodies: anti-OTR (1:500; Abcam, Cambridge, UK), anti-calcitonin gene-related peptide (CGRP; 1:3000; Abcam, Cambridge, UK), anti-c-fos (1:1000; Abcam, Cambridge, UK), anti-PSD-95 (1:1000; Cell Signaling Technology, Massachusetts, USA), anti-syt-1 (1:1000; Bioss, Beijing, China), anti-snap25 (1:4000; Abcam, Cambridge, UK), anti-NR2B (1:1000; Proteintech, Illinois, USA), anti-NR2B phosphor (pNR2B-Y1472; 1:500; Bioss, Beijing, China), anti-NR2B phosphor (pNR2B-Y1252; 1:1000; Abcam Cambridge, UK), anti-AC1 (1:500; Abcam, Cambridge, UK), anti-PKA C-α (1:3000; Cell Signaling Technology, Massachusetts, USA), anti-CREB (1:1000; Wanlei Bio, Shenyang, China), anti-CREB phosphor (pCREB ; 1:5000; Abcam, Cambridge, UK), and anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:5000; Zen-Bioscience, Chengdu, China).

Techniques: Expressing

Journal: The Journal of comparative neurology

Article Title: Three-Dimensional Ultrastructure of Flower-Spray Nerve Endings in the Rat Carotid Sinus.

doi: 10.1002/cne.25654

Figure Lengend Snippet: FIGURE 1 Whole mount preparation of the carotid sinus with double immunofluorescence for P2 × 3 (green) and Syt1 (red). (a) Low magnification view of the carotid sinus showing P2 × 3-immunoreactive flower-spray nerve endings. Syt1 immunoreactivity is shown in flower-spray endings and in the network of varicose nerve fibers. (b and c) Three-dimensional view of the basal surface of the terminal part of the flower-spray ending indicated in rectangle in Panel a. Arrows indicate thick parent axon for the ending. Panel b shows flower-spray endings could be distinguished from network of thin varicose nerve fibers with Syn1 immunoreactivity. (d–f) Higher magnification view of the rectangle in Panel a. (d) Punctate P2 × 3 immunoreactivity is shown in the terminal part of the endings. (e) Syt1 immunoreactivity is shown as smaller dots in the terminal parts. (f) The merged figure shows that P2 × 3 and Syt1 immunoreactivities are distinct from each other.

Article Snippet: A mouse monoclonal anti-Syt1 antibody (clone ASV48, MAB4364, R&D Systems, Minneapolis, MN, USA; RRID AB_2199304) was raised against the rat brain synaptic plasmamembrane (Matthew, Tsavaler, and Reichardt 1981).

Techniques: Immunofluorescence